Postharvest crown chemical treatments and application timing on internal browning in four pineapple clones

Authors

DOI:

https://doi.org/10.32945/atr47212.2025

Keywords:

Cold storage, Ion leakage, Phenol, Physiological disorders, Shelf life

Abstract

Pineapple clones differ in their sensitivity to internal browning (IB), with the Queen group being more sensitive than the Cayenne group. However, there is not much information available on the differences in clone sensitivity between the Cayenne groups. The aim of this research was to study the responses of Smooth Cayenne and MD2 (hybrid) after chemical spraying applied to the pineapple crown on the IB and other fruit qualities. The experimental design used was a 3 factorial completely randomized design (4 x 5 x 2), clone (GP3; HC; GP4; MD2), chemical material (50mg L-1 ABA; 200mg L-1 AsA  [ascorbic acid]; 1 mM jasmonic acid; 2% calcium chloride; control [H 2O]), and application time 2 (0 and 16 days after harvest). The results showed that the MD2 pineapple clone had greater resistance to IB damage than the Smooth Cayenne type. The Smooth Cayenne type itself had different resistance to IB, with the GP3 clone showing the lowest resistance, followed by HC and GP4. Pineapple resistance to IB was followed by endogenous AsA content; MD2 had the highest AsA content. The susceptibility of GP3 pineapple to IB was also characterized by high ion leakage values. The pineapple postharvest crown-coating applications with chemical materials and/or application time effects on Smooth Cayenne and MD2 types were not effective in suppressing IB severity. Therefore, the need for long pineapple storage of up to 46 days with good quality is recommended only for the MD2 clone.

References

Ahmadi bin Thalip, A., Tong, P. S., & Casey, N. (2015). The MD2 “Super Sweet” pineapple (Ananas comosus). Utar Agriculture Science, 1(4), 14–17. http://eprints.utar.edu.my/1982/1/The_MD2_(Super_Sweet)_pineapple_(Ananas_comosus).pdf

Chan, Y. K., Coppens d’Eeckenbrugge, G., & Sanewski, G. M. (2003). Breeding and variety improvement. In D. P. Bartholomew, R. E. Paull, & K. G. Rohrbach (Eds.), The pineapple: Botany, production and uses (pp. 33–56). CAB International. https://www.cabidigitallibrary.org/doi/pdf/10.5555/20083015203

Chandra, D., Widodo, S. E., Kamal, M., & Waluyo, S. (2023a). Effect of storage temperature transfer on the internal browning and other fruit qualities of GP3 and MD2 pineapple clones after postharvest applications of ABA, chitosan and decrowning. IOP Conference Series: Earth and Environmental Science, 1230, Article 012065. https://doi.org/10.1088/1755-1315/1230/1/012065

Chandra, D., Widodo, S. E., Kamal, M., & Waluyo, S. (2023b). Pineapple responses to postharvest applications of ABA, chitosan, and decrowning on internal browning and other fruit qualities. Acta Innovations, 47(6), 64–72. https://doi.org/10.32933/ActaInnovations.47.6

Chandra, D., Widodo, S. E., Kamal, M., & Waluyo, S. (2023c). Postharvest treatments influenced the incidence of internal browning, phenol, ABA, and GA3 contents of two pineapple clones. Acta Innovations, 50(7), 74–80.https://doi.org/10.32933/ActaInnovations.50.7

Dolhaji, N. H., Muhamad, I. I., Ya’akub, H., & Aziz, A. A. (2019). Evaluation of chilling injury and internal browning condition on quality attributes, phenolic content, and antioxidant capacity during sub-optimal cold storage of Malaysian cultivar pineapples. Malaysian Journal of Fundamental and Applied Sciences, 14(4), 456–461.https://doi.org/10.11113/mjfas.v14n4.1072

Farag, K. M., & Nagy, N. M. N. (2012). Effect of pre- and post-harvest calcium and magnesium compounds and their combination treatments on “Anna” apple fruit quality and shelf life. Journal of Horticultural Science & Ornamental Plants, 4(2) ,155–168. https://idosi.org/jhsop/4(2)12/5.pdf

Ko, H. L., Campbell, P. R., Jobin-Décor, M. P., Eccleston, K. L., Graham, M. W., & Smith, M. K. (2006). The introduction of transgenes to control blackheart in pineapple (Ananas comosus L.) cv. Smooth Cayenne by microprojectile bombardment. Euphytica, 150, 387–395. https://doi.org/10.1007/s10681-006-9124-5

Liu, J., He, C., Shen, F., Zhang, K., & Zhu, S. (2017). The crown plays an important role in maintaining quality of harvested pineapple. Postharvest Biology and Technology, 124, 18–24. https://doi.org/10.1016/j.postharvbio.2016.09.007

Luengwilai, K., Beckles, D. M., Roessner, U., Dias, D. A., Lui, V., & Siriphanich, J. (2018). Identification of physiological changes and key metabolites coincident with postharvest internal browning of pineapple (Ananas comosus L.) fruit. Postharvest Biology and Technology, 137, 56–65. https://doi.org/10.1016/j.postharvbio.2017.11.013

Luengwilai, K., Beckles, D. M., & Siriphanich, J. (2016). Postharvest internal browning of pineapple fruit originates at the phloem. Journal of Plant Physiology, 202, 121–133. https://doi.org/10.1016/j.jplph.2016.07.011

Min, K., Chen, K., & Arora, R. A. (2020). A metabolomics study of ascorbic acid-induced in situ freezing tolerance in spinach (Spinacia oleracea L.). Plant Direct, 4(2), e00202. https://doi.org/10.1002/pld3.202

Naradisorn, M., Yuenyongsaen, K., & Setha, S. (2022). Preharvest calcium application maintains antioxidant capacity and postharvest quality of 'Phulae' pineapple. Fruits, The International Journal of Tropical and Subtropical Horticulture, 77(5), 1–8. https://www.cabidigitallibrary.org/doi/10.17660/th2022/021

Nukuntornprakit, O., Chanjirakul, K., van Doorn, W. G., & Siriphanich, J. (2015). Chilling injury in pineapple fruit: Fatty acid composition and antioxidant metabolism. Postharvest Biology and Technology, 99, 20–26. https://doi.org/10.1016/j.postharvbio.2014.07.010

Pusittigul, I., Kondo, S., & Siriphanich, J. (2012). Internal browning of pineapple (Ananas comosus L.) fruit and endogenous concentrations of abscisic acid and gibberellins during low temperature storage. Scientia Horticulturae, 146, 45–51. https://doi.org/10.1016/j.scienta.2012.08.008

Queiroz, C., Lopes, M. L. M., Fialho, E., & Valente-Mesquita, V. L. (2008). Polyphenol oxidase: Characteristics and mechanisms of browning control. Food Reviews International, 24(4), 361–375. https://doi.org/10.1080/87559120802089332

Saleem, M. S., Anjum, M. A., Naz, S., Ali, S., Hussain, S., Azam, M., Sardar, H., Khaliq, G., Canan, I., & Ejaz, S. (2021). Incorporation of ascorbic acid in kitosan-based edible coating improves postharvest quality and storability of strawberry fruits. International Journal of Biological Macromolecules, 189, 160–169. https://doi.org/10.1016/j.ijbiomac.2021.08.051

Souleymane, C., Edwige-Salome, Y. S., Laurent, N. A., Samuel, K. O. K., & Hilaire, K. T. (2019). Effect of potassium fertilization for pineapple on internal browning of fruit in post-harvest conservation. Journal of Agriculture and Crops, 5(6), 100–108. https://doi.org/10.32861/jac.56.100.108

Youryon, P., Supapvanich, S., & Wongs-Aree, C. (2019). Internal browning alleviation of Queen pineapple cv. ‘Sawi’ under cold storage using salicylic acid or abscisic acid peduncle infiltration. The Journal of Horticultural Science and Biotechnology, 94(6), 744–752. https://doi.org/10.1080/14620316.2019.1621684

Yuan, Q., Jiang, Y., Yang, Q., Li, W., Gan, G., Cai, L., Li, W., Qin, C., Yu, C., & Wang, Y. (2024). Mechanisms and control measures of low temperature storage-induced chilling injury to solanaceous vegetables and fruits. Frontiers in Plant Science, 15, 1488666. https://doi.org/10.3389/fpls.2024.1488666

Zhang, Q., Liu, Y., He, C., & Zhu, S. (2015). Postharvest exogenous application of abscisic acid reduces internal browning in pineapple. Journal of Agricultural and Food Chemistry, 63(22), 5313–5320. https://doi.org/10.1021/jf506279x

Zhang, Q., Rao, X., Zhang, L., He, C., Yang, F., & Zhu, S. (2016). Mechanism of internal browning of pineapple: The role of gibberellins catabolism gene (AcGA2ox) and GAs. Scientific Reports, 6, 33344. https://doi.org/10.1038/srep33344

Submitted

2025-10-03

Accepted

2025-11-07

Published

2025-12-10

How to Cite

Chandra, D., Widodo, S. E., Kamal, M., Waluyo, S., & Setiawan, K. (2025). Postharvest crown chemical treatments and application timing on internal browning in four pineapple clones . Annals of Tropical Research, 47(2), 176–186. https://doi.org/10.32945/atr47212.2025

Issue

Section

Original Research Article

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